ProSci

Smad9 Antibody

Product Code:
 
PSI-46-383
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-383 (0.3ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-383 (0.3ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-46-383-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)] , HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B , MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SMAD9 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Background References:
  • Shi Y, Massague J. Mechanisms of TGF-beta signaling from cell membrane to the nucleus. Cell. 2003 Jun 13;113(6):685-700. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-HSEPLMPHNATYPD
NCBI Gene ID #:
4093
NCBI Official Name:
SMAD family member 9
NCBI Official Symbol:
SMAD9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005896.1
Protein GI Number:
5174519
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is not expected to cross-react with SMAD1 and SMAD5.
Swissprot #:
O15198