Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
1 / 2
2 / 2
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
H2AX, H2A.X, H2A/X, MCH6, H2AFX, H2a/x, HIST5-2AX
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
- Yaneva M, et al. (2005) Nucleic Acids Res. 33 (16): 5320-5330. Tsukuda T, et al. (2006) Nature. Author manuscript; available in PMC 2006 March 6
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Histone H2A.X (Ab-139) antibody was raised against a peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
3014
H2A histone family, member X
H2AFX
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
NP_002096.1
4504253
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Cell Cycle
This antibody detects endogenous level of total Histone H2A.X protein.
P16104