ProSci

Thioredoxin Monoclonal Antibody

Product Code:
 
PSI-32-225
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1H6H6
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Thioredoxin (TRX) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.
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Western blot analysis using Trx monoclonal antibody against four different fusion protein with truncated Trx tag.

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PSI-32-225-0.1mL0.1mL£452.00
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Further Information

Additional Names:
CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, NKR-P1A, NKR, CD161, NKR-P1, hNKR-P1A
Application Note:
Dilution
Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Thioredoxin (TRX) is a small ubiquitous protein (MW12 kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site.This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation.The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.
Background References:
  • Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985).
  • Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988).
  • Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human Thioredoxin (TRX).
NCBI Gene ID #:
3820
NCBI Official Name:
killer cell lectin-like receptor subfamily B, member 1
NCBI Official Symbol:
KLRB1
NCBI Organism:
Homo sapiens
Protein Accession #:
Q12918
Protein GI Number:
74722301
Swissprot #:
Q12918
User NOte:
Optimal dilutions for each application to be determined by the researcher.