ProSci

NANOG Antibody

Product Code:
 
PSI-45-932
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-932 (0.05ug/ml) staining of HeLa (nuclear) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-932 (0.05ug/ml) staining of HeLa (nuclear) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-932-0.1mg0.1mg£626.00
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Further Information

Additional Names:
NANOG, Nanog homeobox, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.05-0.1ug/ml. An additional band of approx. 37kDa is observed and is consistent with the observations of higher molecular weight bands regularly found with antibodies from other sources.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia. Recommended concentration, 1-2ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant NANOG captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. This antibody was reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB06860.
Background References:
  • Yasuda SY, Tsuneyoshi N, Sumi T, Hasegawa K, Tada T, Nakatsuji N, Suemori H. NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer. Genes Cells. 2006 Sep;11(9):1115-23.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-DLFLNYSMNMQPED
NCBI Gene ID #:
79923
NCBI Official Name:
Nanog homeobox
NCBI Official Symbol:
NANOG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079141.2
Protein GI Number:
13376298
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Stem Cell
SPECIFICITY:
.
Swissprot #:
Q9H9S0