Further Information
CALB1, calbindin 1, 28kDa, CALB, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, calbindin, calbindin 1, (28kD), calbindin D28, vitamin D-dependent calcium-binding protein, avian-type
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
- Kusakabe KT, Abe H, Kondo T, Kato K, Okada T, Otsuki Y.DNA microarray analysis in a mouse model for endometriosis and validation of candidate factors with human adenomyosis.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
The immunogen for this antibody is: C-KTFVDQYGQRDDGK
793
calbindin 1, 28kDa
CALB1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 26kDa
NP_004920.1
115394
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer,Immunology,Obesity,
Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
P05937