Chemodex

DAF-2 Solution

Product Code:
 
CDX-D0584
Product Group:
 
Dyes, Stains, and Probes
Supplier:
 
Chemodex
Regulatory Status:
 
RUO
Shipping:
 
Ambient
Storage:
 
+4°C
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Chemical Structure

Chemical Structure

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CDX-D0584-M0011 mg£487.00
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Further Information

Alternate Names/Synonyms:
4,5-Diaminofluorescein
Appearance:
Liquid.
CAS:
205391-01-1
Concentration:
5mM in DMSO (1mg in 0.55ml DMSO)
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Keep cool and dry.Protect from light.
Hazards:
H302
InChi:
InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChiKey:
LTYUPYUWXRTNFQ-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 205391-01-1. Formula: C20H14N2O5. MW: 362.34. Synthetic. DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: lambdaex 505nm; lambdaem 518nm in 0.1M Tris pH8.0.
MDL:
MFCD05865100
Molecular Formula:
C20H14N2O5
Molecular Weight:
362.34
Package Type:
Vial
Precautions:
P264, P270, P301, P312, P330
Product Description:
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells. Spectral data: lambdaex 505nm; lambdaem 518nm in 0.1M Tris pH8.0.
Purity:
>95% (HPLC)
Signal Word:
Warning
SMILES:
NC1=CC2=C(C=C1N)C1(OC2=O)C2=CC=C(O)C=C2OC2=C1C=CC(O)=C2
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.

References

(1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006) | (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003) | (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999) | (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998) | (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)