Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
Interleukin-33; IL-1F11; NF-HEV
ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
1 mg/ml
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant mouse IL-33.
77125
interleukin 33
Il33
Mus musculus
Liquid
68565902
Q8BVZ5
Innate Immunity
Recognizes mouse IL-33. Detects a band of ~18kDa by Western blot. Does not cross-react with human IL-33.
Q8BVZ5
Optimal dilutions for each application to be determined by the researcher.