Further Information
GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, ERAS, HRAS2, HRASP
For IF starting dilution is: 1:100
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
- References for protein:
- Kameda,T., Stem Cells 23 (10), 1535-1540 (2005)
- Takahashi,K., Nature 423 (6939), 541-545 (2003)
- Miyoshi,J., Nucleic Acids Res. 12 (4), 1821-1828 (1984)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
3266
GTPase ERas
ERAS
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Q7Z444
74723320
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Cancer,Cell Cycle,Signal Transduction
Q7Z444
Optimal dilutions for each application to be determined by the researcher.