ProSci

INDO Antibody

Product Code:
 
PSI-61-459
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-INDO Antibody in 293 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with INDO antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-INDO Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with INDO antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-459-400ul400ul£626.00
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Further Information

Additional Names:
Indoleamine 2,3-dioxygenase 1, IDO-1, Indoleamine-pyrrole 2,3-dioxygenase, IDO1, IDO, INDO
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Gamma-interferon (IFNG; MIM 147570) has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and Chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase (INDO; EC 1.13.11.52). This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine.[supplied by OMIM]
Background References:
  • Maghzal,G.J., J. Biol. Chem. 283 (18), 12014-12025 (2008)
  • Chauhan,N., Biochemistry 47 (16), 4761-4769 (2008)
  • Scheler,M., Am. J. Pathol. 171 (6), 1936-1943 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This INDO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-403 amino acids from the C-terminal region of human INDO.
NCBI Gene ID #:
3620
NCBI Official Name:
Indoleamine 2,3-dioxygenase 1
NCBI Official Symbol:
IDO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P14902
Protein GI Number:
123948
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P14902
User NOte:
Optimal dilutions for each application to be determined by the researcher.