ProSci

ACOT11 Antibody

Product Code:
 
PSI-55-048
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in HepG2 cell line lysates (35ug/lane).
2 / 3
ACOT11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in HepG2 cell line lysates (35ug/lane).
ACOT11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-048-400ul400ul£626.00
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Further Information

Additional Names:
Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, 312-, Acyl-CoA thioester hydrolase 11, Adipose-associated thioesterase, Brown fat-inducible thioesterase, BFIT, ACOT11, BFIT, KIAA0707, THEA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants.
Background References:
  • Kirkby, B., et al. Prog. Lipid Res. 49(4):366-377(2010)
  • Hunt, M.C., et al. J. Lipid Res. 46(9):2029-2032(2005)
  • Adams, S.H., et al. Biochem. J. 360 (PT 1), 135-142 (2001) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ACOT11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-575 amino acids from the C-terminal region of human ACOT11.
NCBI Gene ID #:
26027
NCBI Official Name:
Acyl-coenzyme A thioesterase 11
NCBI Official Symbol:
ACOT11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q8WXI4
Protein GI Number:
21363000
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q8WXI4
User NOte:
Optimal dilutions for each application to be determined by the researcher.