Further Information
Ubiquitin carboxyl-terminal hydrolase 13, Deubiquitinating enzyme 13, Isopeptidase T-3, ISOT-3, Ubiquitin thioesterase 13, Ubiquitin-specific-processing protease 13, USP13, ISOT3
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
- Puente, X.S., et al., Nat. Rev. Genet. 4(7):544-558 (2003).
- D'Andrea, A., et al., Crit. Rev. Biochem. Mol. Biol. 33(5):337-352 (1998).
- Timms, K.M., et al., Gene 217 (1-2), 101-106 (1998).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine
This USP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-419 amino acids from the Central region of human USP13.
8975
Ubiquitin carboxyl-terminal hydrolase 13
USP13
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Q92995
209572692
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Cell Cycle
Q92995
Optimal dilutions for each application to be determined by the researcher.