ProSci

EIF2B5 Antibody

Product Code:
 
PSI-56-847
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in mouse testis tissue lysates (35ug/lane).
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EIF2B5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse testis tissue lysates (35ug/lane).
EIF2B5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-56-847-400ul400ul£626.00
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Further Information

Additional Names:
Translation initiation factor eIF-2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2B5, EIF2BE
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Background References:
  • van der Lei, H.D., et al. Neurology 75(17):1555-1559(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Pronk, J., et al. Mult. Scler. 14(8):1123-1126(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This EIF2B5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human EIF2B5.
NCBI Gene ID #:
8893
NCBI Official Name:
Translation initiation factor eIF-2B subunit epsilon
NCBI Official Symbol:
EIF2B5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
80 kDa
Protein Accession #:
Q13144
Protein GI Number:
160359049
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q13144
User NOte:
Optimal dilutions for each application to be determined by the researcher.