NSJ Bioreagents

Collagen II Antibody / COL2A1

Product Code:
 
NSJ-R31259
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the Collagen Type 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of Collagen Type 2 antibody and Lane 1: COLO320; 2: HeLa; 3: MCF-7; 4: A549; 5: Jurkat lysate. Expected/observed size ~140KD
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Western blot testing of Collagen Type 2 antibody and Lane 1: COLO320; 2: HeLa; 3: MCF-7; 4: A549; 5: Jurkat lysate. Expected/observed size ~140KD

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NSJ-R31259-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Collagen Type 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Collagen type 2 alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocytes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human COL2A1 (AFAGLGPREKGPDPLQYMRA) was used as the immunogen for this Collagen Type 2 antibody.
Limitation:
This Collagen Type 2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02458