NSJ Bioreagents

GRP94 Antibody

Product Code:
 
NSJ-R31967
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the GRP94 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 9
Immunofluorescent staining of FFPE human A431 cells with GRP94 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
IHC staining of FFPE human lung cancer with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human intestinal cancer with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE mouse brain with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE rat brain with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE human tonsil with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) A431, 4) Caco-2, 5) ThP-1, 6) Caco-2, 7) HepG2 and 8) HL60 cell lysate with GRP94 antibody. Expected molecular weight : 92~96 kDa.
8 / 9
Western blot testing of rat 1) PC-12, 2) RH35, 3) C6 and mouse 4) HEPA1-6, 5) NIH 3T3, 6) RAW264.7, 7) SP2/0 and 8) ANA-1 cell lysate with GRP94 antibody. Expected molecular weight : 92~96 kDa.
9 / 9
Flow cytometry testing of human ThP-1 cells with GRP94 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRP94 antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with GRP94 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GRP94 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) A431, 4) Caco-2, 5) ThP-1, 6) Caco-2, 7) HepG2 and 8) HL60 cell lysate with GRP94 antibody. Expected molecular weight : 92~96 kDa.
Western blot testing of rat 1) PC-12, 2) RH35, 3) C6 and mouse 4) HEPA1-6, 5) NIH 3T3, 6) RAW264.7, 7) SP2/0 and 8) ANA-1 cell lysate with GRP94 antibody. Expected molecular weight : 92~96 kDa.
Flow cytometry testing of human ThP-1 cells with GRP94 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GRP94 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-R31967-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the GRP94 antibody should be determined by the researcher.
Description:
GRP94 (94 kDa glucose-regulated protein), also called Endoplasmin, is a chaperone protein that in humans is encoded by the HSP90B1 gene. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 43-221 of human GRP94 were used as the immunogen for the GRP94 antibody.
Limitation:
This GRP94 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14625