NSJ Bioreagents

HDAC5 Antibody

Product Code:
 
NSJ-R31301
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Storage:
 
After reconstitution, the HDAC5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of HDAC5 antibody and Lane 1: HeLa; 2: COLO320 cell lysate. Expected/observed size ~122KD
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Western blot testing of HDAC5 antibody and Lane 1: HeLa; 2: COLO320 cell lysate. Expected/observed size ~122KD

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NSJ-R31301-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HDAC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Histone deacetylase 5 is an enzyme that in humans is encodes by the HDAC5 gene. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. This gene is mapped to 17q21.31. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. HDAC5 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2(MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is though to be associated with colon cancer.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Histone deacetylase 5 (YKKLFSDAQPLQPLQVYQA) was used as the immunogen for this HDAC5 antibody.
Limitation:
This HDAC5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UQL6