NSJ Bioreagents

LEF1 Antibody / Lymphoid enhancer-binding factor 1 / TCF1 alpha

Product Code:
 
NSJ-V5624
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LEF1/9403R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant LEF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human tonsil tissue with recombinant LEF1 antibody (clone LEF1/9403R). Strong nuclear staining of non-germinal center of cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant recombinant LEF1 antibody (clone LEF1/9403R) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human tonsil tissue with recombinant LEF1 antibody (clone LEF1/9403R). Strong nuclear staining of non-germinal center of cells observed. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant recombinant LEF1 antibody (clone LEF1/9403R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V5624-20UG20 ug£264.00
Quantity:
NSJ-V5624-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the recombinant LEF1 antibody should be determined by the researcher.
Description:
Lymphoid enhancer-binding factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant fragment (within amino acids 1-200) of human LEF1 protein was used as the immunogen for the recombinant LEF1 antibody.
Limitation:
This recombinant LEF1 antibody is available for research use only.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q9UJU2