Nile Red
Product Code:
CDX-N0107
CDX-N0107
Regulatory Status:
RUO
RUO
Shipping:
AMBIENT
AMBIENT
Storage:
-20°C
-20°C
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
CDX-N0107-G001 | 1 g | £194.00 |
Quantity:
CDX-N0107-G005 | 5 g | £743.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
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Further Information
Alternate Names/Synonyms:
Nile Blue A Oxazone; Phenoxazone 9; 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Appearance:
Green to brown powder.
CAS:
7385-67-3
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChiKey:
VOFUROIFQGPCGE-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 7385-67-3. Formula: C20H18N2O2. MW: 318.4. Synthetic. Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm).
MDL:
MFCD00011639
Molecular Formula:
C20H18N2O2
Molecular Weight:
318.4
Package Type:
Vial
Product Description:
Uncharged, hydrophobic, photostable fluorescent probe that strongly fluoresces bright red in hydrophobic (lipid-rich) environments, but is almost nonfluorescent in water. This lipophilic stain is commonly used for the detection of intracellular lipid droplets in cells such as adipocytes by fluorescence microscopy and flow cytometry. It has been shown to detect the exposure or formation of new hydrophobic surfaces induced by ligand binding to calmodulin, oligomerization of melittin, or unfolding of ovalbumin during early thermal denaturation. This product has been employed to stain cell structures, as well as to visualize and localize colloidal drug carriers. Has been used to stain lipophilic proteins that have been separated by SDS-PAGE electrophoresis or to detect sphingolipids on thin-layer chromatograms. The fluorescence is unaffected between pH 4.5 and 8.5 and may be used in dilute concentrations to investigate hydrophobic protein sites. Spectral properties: Intracellular fat vacuoles, filled with neutral lipids such as cholesterol, lipoproteins and triglycerides will fluoresce green (Abs/Em =485/525) while polar lipids, such as phospholipids, will fluoresce red (Abs/Em = 552/636 nm). Extinction coeff. 0.60 ? 0.05 (0.01g/l; 0,5cm).
Purity:
>98% (HPLC)
SMILES:
CCN(CC)C1=CC=C2N=C3C(OC2=C1)=CC(=O)C1=C3C=CC=C1
Solubility Chemicals:
Soluble in chloroform.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
Documents
References
(1) P. Greenspan, et al.; J. Cell Biol. 100, 965 (1985) | (2) S.D. Fowler & P. Greenspan; J. Histochem. Cytochem. 33, 833 (1985) | (3) D.L. Sackett & J. Wolff; Anal. Biochem. 167, 228 (1987) | (4) F.J. Alba, et al; Biotechniques 21, 625 (1996) | (5) A. Lamprecht, et al.; J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 787, 415 (2003) | (6) A. Makino, et al.; Biochem. 45, 4530 (2006) | (7) T. Werner, et al.; Cytometry A 69, 200 (2006) | (8) B.W. Halstead, et al.; J. Appl. Toxicol. 26, 169 (2006) | (9) R. Varghese, et al.; Chemistry 16, 9040 (2010)
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