NSJ Bioreagents

ZO-2 Antibody

Product Code:
 
NSJ-R31060
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the ZO-2 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with ZO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
Immunofluorescent staining of FFPE human A431 cells with ZO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
IHC staining of FFPE human intestinal cancer tissue with ZO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human HaCaT, 5) human MCF7, 6) human U-2 OS, 7) human HeLa, 8) human PC-3, 9) rat liver, 10) rat RH35, 11) mouse liver and 12) mouse HEPA1-6 cell lysate with ZO-2 antibody. Expected molecular weight: 131-160 kDa.
5 / 5
Flow cytometry testing of human MCF7 cells with ZO-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZO-2 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with ZO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A431 cells with ZO-2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human intestinal cancer tissue with ZO-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human HaCaT, 5) human MCF7, 6) human U-2 OS, 7) human HeLa, 8) human PC-3, 9) rat liver, 10) rat RH35, 11) mouse liver and 12) mouse HEPA1-6 cell lysate with ZO-2 antibody. Expected molecular weight: 131-160 kDa.
Flow cytometry testing of human MCF7 cells with ZO-2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZO-2 antibody.

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CodeSizePrice
NSJ-R31060-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ZO-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Tight Junction Protein 2, also known as Zona occludens 2 or ZO-2 is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs), or zonulae occludentes, belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al.(1994) mapped the gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. It lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al.(1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al.(2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth(P0).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A synthetic peptide corresponding to human Zona occludens 2 (SEHSKRGYYGQSARYRDTEL) was used as the immunogen for this ZO-2 antibody.
Limitation:
This ZO-2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9UDY2