Agarose Bead Technologies (ABT) - Breaking the Barriers in Bioseparation with High-Perfomance Chromatography Resins - Distributed by Caltag Medsystems

Agarose Bead Technologies (ABT) is a premier manufacturer of high-quality agarose resins with locations in the US and Europe. The agarose resins, manufactured under a rigorous quality management system (QMS), are used in chromatographic purification, separation, or immobilisation processes of biomolecules including proteins, antibodies, enzymes, and oligonucleotides in discovery, development, and cGMP production of biopharmaceuticals.

ABT owns a proprietary agarose-based resin technology to develop a wide range of non-activated agarose beads for SEC and activation procedures, activated agarose beads for Affinity and IEX chromatography, as well as pre-activated resins for coupling of affinity ligands.

ABT custom products are made in compliance with client specifications on a large scale – a unique capability in the industry. ABT is an ISO 9001:2015 certified company with cGMP facilities operated by experts with in-depth knowledge of agarose.

See the Products Available:

Size Exclusion Chromatography

Ion Exchange Chromatography

Affinity Chromatography

Size Exclusion Chromatography

ABT offers a wide range of plain and crosslinked agarose beads with different agarose concentrations (2, 4, 6, 8, & 10%) in different particle distributions:

Standard (50-150µm),
Fine (20-50µm),
and Macro (150-350µm).

ABT has also developed Rapid Run™ which are based on highly crosslinked 4% and 6% agarose matrices used for industrial scale separations.

See all Size Exclusion Chromatography products

Ion Exchange Chromatography

Ion Exchange Chromatography (IEX) separates biomolecules based on their net surface charge. Biomolecules will provide different interaction properties depending on the net charge, density, and surface charge distribution.

Depending on the working pH, the biomolecule will exhibit interaction to anion or cation exchanger media. Therefore, at a pH above its isoelectric point (pI), the biomolecule will interact with a positively charged resin (anion exchanger), and at a pH below its pI, the biomolecule will show affinity to a negatively charged resin (cation exchanger).

See all Ion Exchange Chromatography products

Affinity Chromatography

Affinity Chromatography is a technique that separates tagged proteins and other biomolecules using biological interactions. This technique has high selectivity and is widely used to obtain proteins with high purity at high yields. ABT manufactures a variety of activated beads used for His-tag, Antibody, GST, and Avidin/Streptavidin Purifications.

See all Affinity Chromatography products

Affinity Coupling

ABT offers a great range of pre-activated resins that are designed to couple ligands via stable & uncharged covalent linkages that minimise leakage of the affinity ligand and reduce non-specific binding.

Two different chemistries of pre-activated beads are available: Glyoxal and Aminoethyl beads.

The choice of Glyoxal or Aminoethyl will depend on the ligand to be immobilised, the accessibility of the reactive groups and the direction/orientation required for the binding to the support.

See all Affinity Coupling products

Magnetics

Magnetic separation is a fast and broad use method for a variety of biomolecules bench-top purifications.

Magnetic agarose resins offer a simplistic purification step due to their ferrimagnetic formulation properties; the same ligand coupling chemistry as conventional agarose beads, being suitable for a wide range of applications.

ABT offers plain agarose beads (4% concentration) with the option of two particle sizes for magnetic separation procedures.

See all Magnetics

Columns

ABT offers chromatography accessories for working with small volumes of resins. The selection will depend on the equipment available in the purification step and the quantity of sample that needs to be purified.

These single-use chromatography tools, which simplify the use of the resins and make the purification process simple and rapid.

See all Columns

Customised Agarose Resins

The customisation of agarose resins is ABT's flagship service, and they develop various custom resins that meet the unique challenges of their clients.

ABT's custom product range is the result of their extensive research, expertise, and production technology capabilities with precision control of various parameters. Their custom range also includes agarose beads of various sizes with appropriate separation properties.

Parameters that can be specified:

Size of beads
Degree of crosslinking
Pore size
Concentration of agarose
Spacer arm length
Type of activation
Density of active groups

Caltag Medsystems is here to support you every step of the way with your ABT custom projects.

Documents

Category	Title
Resins Documents	Glyoxal Agarose Resins Infographic	View
Pre-activated agarose resins designed for the covalent coupling of ligands containing primary amino groups.
Resins Documents	GLYOXAL AGAROSE	View
Base beads for affinity chromatography resins
Supplier Introduction Documents	Agarose Bead Technologies	View
PREMIER MANUFACTURER OF HIGH-QUALITY AGAROSE RESINS.
Service Documents	Custom Services	View
The perfect resin for your application.
Resins Documents	APPLICATION NOTE: Protein G Agarose 4 Rapid Run™ Affinity Chromatography Resin Benchmarking studies	View
Protein G is a well-established ligand for affinity chromatography resins used for isolation and characterization of immunoglobulins. Protein G binds to the Fc region of mammalian IgGs thus exhibiting high affinity for many IgG subclasses including human, rabbit, mouse, rat, sheep, pig, horse, cow or goat. In contrast to protein A, protein G binds all human and mouse IgG subclasses, including human IgG3 and mouse IgG1 as well as all rat IgG subclasses. Furthermore, protein G also binds to CH1 portion of the Fab region making this ligand well-suited for the purification of more complex constructs such as bispecific antibodies.
Resins Documents	APPLICATION NOTE: Protein G Agarose 4 Rapid Run™ Affinity Chromatography Resin	View
Protein G Agarose 4 Rapid Run™ is an affinity chromatography resin developed by Agarose Bead Technologies for purification of immunoglobulins.
Resins Documents	Advancements in Resin Development - Oligonucleotide Purification with IEX Matrix	View
Ion Exchange Chromatography (IEX) is one of the most frequently used techniques for biotherapeutics separation since it separates biomolecules based on their net surface charge. For oligonucleotide purification, anion exchange chromatography (AEX) is an effective technique that can be helpful for the removal of incomplete or erroneous sequences, resulting in high purity and yield. However, there is always a need to improve the purity and yield of the purification process with a new purification matrix. In this study, two new anion-exchange agarose resins were developed by Agarose Bead Technologies (ABT), Q10F Rapid RunTM High Sub (QRR10FPT1) and QRRPT1 Rapid RunTM High Sub Agarose Bead Fine (QRRFPT1), which greatly improved the loading capacity, purification yield, and purity of a crude mRNAs oligonucleotide.
Resins Documents	Improving MAbs Purification in Downstream Processes	View
Antibodies are high-added-value molecules and as the biotechnological industry grows, it becomes necessary to find new solutions that increase the purity and yield of their purification processes. One alternative is the use of Protein G which has a high affinity for the Fc region and CH1 portion of monoclonal IgG-type antibodies. Protein G agarose resins have gained interest in this solution because of its great performance and capabilities. Here ABT presents a newly developed protein G agarose resin with an improved binding affinity. The study focused on the comparison of binding capacities, yields, and purities for targets purified from different real feed samples. The targets included IgG from fresh mouse serum and pure rat IgG. All resins used were agarose-based.
Catalogue Videos	Agarose Bead Technologies Corporate video	View
ABT offers a wide range of non-activated agarose resins for Size Exclusion and activation procedures, as well as activated agarose beads for Affinity Chromatography, Ion Exchange Chromatography, and Pre-activated resins for coupling of affinity ligands (immobilisation).
Catalogue Documents	ABT Catalogue - Categories	View
BREAKING THE BARRIERS IN BIOSEPARATIONS