NSJ Bioreagents

Alpha Actin Antibody / ACATA1 (skeletal muscle)

Product Code:
 
NSJ-RQ4494
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C-1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Alpha Actin antibody at -200.
1 / 3
Western blot testing of human 1) A431 and 2) MCF7 lysate with Alpha Actin antibody at 0.5ug/ml. Expected molecular weight: 42-45 kDa.
2 / 3
IHC testing of FFPE mouse muscle with Alpha Actin antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Immunofluorescent staining of human HeLa cells with Alpha Actin antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human 1) A431 and 2) MCF7 lysate with Alpha Actin antibody at 0.5ug/ml. Expected molecular weight: 42-45 kDa.
IHC testing of FFPE mouse muscle with Alpha Actin antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human HeLa cells with Alpha Actin antibody (green) and DAPI nuclear stain (blue).

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NSJ-RQ4494-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the Alpha Actin antibody should be determined by the researcher.
Description:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide from human Alpha Actin was used as the immunogen for the Alpha Actin antibody.
Limitation:
This Alpha Actin antibody is available for research use only.
Localization:
Cytoplasm, cytoskeleton
Purity:
Protein A affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P68133