NSJ Bioreagents

Collagen XVII Antibody / COL17A1

Product Code:
 
NSJ-RQ4497
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AA-3
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Collagen XVII antibody at -200.
1 / 3
Western blot testing of human skin lysate with Collagen XVII antibody at 0.5ug/ml. Expected molecular weight ~120 kDa and ~180 kDa.
2 / 3
IHC testing of FFPE mouse skin with Collagen XVII antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Immunofluorescent staining of human A431 cells with Collagen XVII antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of human skin lysate with Collagen XVII antibody at 0.5ug/ml. Expected molecular weight ~120 kDa and ~180 kDa.
IHC testing of FFPE mouse skin with Collagen XVII antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human A431 cells with Collagen XVII antibody (green) and DAPI nuclear stain (blue).

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NSJ-RQ4497-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the Collagen XVII antibody should be determined by the researcher.
Description:
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide from human Collagen XVII was used as the immunogen for the Collagen XVII antibody.
Limitation:
This Collagen XVII antibody is available for research use only.
Localization:
Cytoplasmic, membrane, cell junction
Purity:
Protein A affinity
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UMD9