NSJ Bioreagents

NeuN Antibody / Fox3

Product Code:
 
NSJ-RQ4501
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AO-18
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NeuN antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of human SH-SY5Y cells with NeuN antibody (green) and DAPI nuclear stain (blue).
2 / 3
IHC testing of FFPE human pancreas with NeuN antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Western blot testing of human fetal brain lysate with NeuN antibody at 0.5ug/ml. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).
❮❯

Immunofluorescent staining of human SH-SY5Y cells with NeuN antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human pancreas with NeuN antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human fetal brain lysate with NeuN antibody at 0.5ug/ml. Expected molecular weight: 46-48 kDa (two isoforms may be visualized).

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NSJ-RQ4501-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the NeuN antibody should be determined by the researcher.
Description:
RNA binding protein fox-1 homolog 3 is a RNA-binding protein that regulates alternative splicing events. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide from human NeuN was used as the immunogen for the NeuN antibody.
Limitation:
This NeuN antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A affinity
Species Reactivity :
Human
Uniprot #:
A6NFN3