NSJ Bioreagents

Beta Catenin Antibody

Product Code:
 
NSJ-RQ4508
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
1F6
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Beta Catenin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent testing of FFPE human A431 cells with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 6
Flow cytometry testing of human SiHa cells with Beta Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Catenin antibody.
3 / 6
IHC testing of FFPE human breast cancer with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 6
IHC testing of FFPE human breast cancer with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 6
Western blot testing of human 1) HeLa, 2) COLO-320, 3) HepG2, 4) MCF7, 5) SW620, 6) 22RV1 and 7) Jurkat lysate with Beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
6 / 6
ICC testing of human A549 cells with Beta Catenin antibody.
❮❯

Immunofluorescent testing of FFPE human A431 cells with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human SiHa cells with Beta Catenin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta Catenin antibody.
IHC testing of FFPE human breast cancer with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human breast cancer with Beta Catenin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) HeLa, 2) COLO-320, 3) HepG2, 4) MCF7, 5) SW620, 6) 22RV1 and 7) Jurkat lysate with Beta Catenin antibody. Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.
ICC testing of human A549 cells with Beta Catenin antibody.

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CodeSizePrice
NSJ-RQ4508-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunocytochemistry: 2-4ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Beta Catenin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human beta Catenin recombinant protein (amino acids A2-K233) was used as the immunogen for the Beta Catenin antibody.
Limitation:
This Beta Catenin antibody is available for research use only.
Localization:
Cell surface, cytoplasmic, cell junctions, nuclear
Purity:
Protein G affinity
Species Reactivity :
Human
Uniprot #:
P35222