NSJ Bioreagents

Caspase-2 Antibody

Product Code:
 
NSJ-RQ4542
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Caspase-2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human colon cancer with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
2 / 5
IHC staining of FFPE human lung cancer with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 5
IHC staining of FFPE mouse intestine with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 5
IHC staining of FFPE rat spleen with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
5 / 5
Western blot testing of human 1) PANC-1, 2) 22RV1 and 3) SGC-7901 cell lysate with Caspase-2 antibody at 0.5ug/ml. Predicted molecular weight: ~47 kDa (full), ~31 kDa (large+small subunit), ~20 kDa (large subunit).
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IHC staining of FFPE human colon cancer with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE mouse intestine with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat spleen with Caspase-2 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human 1) PANC-1, 2) 22RV1 and 3) SGC-7901 cell lysate with Caspase-2 antibody at 0.5ug/ml. Predicted molecular weight: ~47 kDa (full), ~31 kDa (large+small subunit), ~20 kDa (large subunit).

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CodeSizePrice
NSJ-RQ4542-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the Caspase-2 antibody should be determined by the researcher.
Description:
Caspase-2, which is involved in stress-induced apoptosis, is recruited into a large protein complex, the molecular composition of which remains elusive. activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD. Increased PIDD expression resulted in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli. Caspase-2 acts both as a positive and negative cell death effector, depending upon cell lineage and stage of development.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids G170-T325 from the human protein were used as the immunogen for the Caspase-2 antibody. This sequence is from the large subunit.
Limitation:
This Caspase-2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42575