NSJ Bioreagents

NOVA1 Antibody

Product Code:
 
NSJ-RQ4592
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the NOVA1 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 12
IHC staining of FFPE mouse brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 12
IHC staining of FFPE mouse brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE rat brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE rat brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of frozen human placental tissue with NOVA1 antibody.
6 / 12
IHC staining of FFPE human liver cancer tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human colorectal adenocarcinoma tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE human breast cancer tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
Immunofluorescent staining of FFPE human U-2 OS cells with NOVA1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 12
Immunofluorescent staining of FFPE human A549 cells with NOVA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
11 / 12
Western blot testing of 1) human PC-3, 2) human A549, 3) human U-87 MG, 4) human ThP-1, 5) rat brain and 6) mouse brain tissue lysate with NOVA1 antibody at 0.5ug/ml. Predicted molecular weight ~52 kDa.
12 / 12
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with NOVA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA1 antibody.
❮❯

IHC staining of FFPE mouse brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen human placental tissue with NOVA1 antibody.
IHC staining of FFPE human liver cancer tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with NOVA1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with NOVA1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with NOVA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human PC-3, 2) human A549, 3) human U-87 MG, 4) human ThP-1, 5) rat brain and 6) mouse brain tissue lysate with NOVA1 antibody at 0.5ug/ml. Predicted molecular weight ~52 kDa.
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with NOVA1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA1 antibody.

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CodeSizePrice
NSJ-RQ4592-100ug100ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (recombinant human protein)
Application Note:
Optimal dilution of the NOVA1 antibody should be determined by the researcher.
Description:
RNA-binding protein Nova-1 is a protein that in humans is encoded by the NOVA1 gene. It is mapped to 14q12. This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids T416-V509 from the human protein were used as the immunogen for the NOVA1 antibody.
Limitation:
This NOVA1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51513