NSJ Bioreagents

NOX5 Antibody

Product Code:
 
NSJ-RQ4593
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NOX5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
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Western blot testing of human 1) HeLa, 2) placenta, 3) SCG-7901, 4) Jurkat and 5) THP-1 lysate with NOX5 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa.
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Western blot testing of human 1) HeLa, 2) placenta, 3) SCG-7901, 4) Jurkat and 5) THP-1 lysate with NOX5 antibody at 0.5ug/ml. Predicted molecular weight: ~86 kDa.

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NSJ-RQ4593-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the NOX5 antibody should be determined by the researcher.
Description:
NOX5 (Nadph Oxidase 5) is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. It is found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, NOX5 was identified as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells (HC), but not normal B cells.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E13-F197 from the human protein were used as the immunogen for the NOX5 antibody.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q96PH1