ProSci

Biotin Antibody [SB58c] (HRP)

Product Code:
 
PSI-99-182
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[SB58c]
Regulatory Status:
 
RUO
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Store vial at 2-8˚C
1 / 1
Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.
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Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.

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PSI-99-182-1mL1mL£524.00
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Further Information

Application Note:
Biotin Antibody [SB58c] for use in ELISA and western blotting assays.
Background:
Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.
Buffer:
Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Concentration:
Lot specific
Conjugate:
HRP
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-KLH
Physical State:
Liquid
Research Area:
Obesity,Signal Transduction
SPECIFICITY:
Biotin
User NOte:
Optimal dilutions for each application to be determined by the researcher.