ProSci

CD3 Antibody [B-B12]

Product Code:
 
PSI-33-274
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
B-B12
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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SDS-PAGE Analysis of Purified, BSA-Free CD3 Antibody (clone B-B12). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free CD3 Antibody (clone B-B12). Confirmation of Integrity and Purity of the Antibody.

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PSI-33-274-100ug100ug£765.00
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Further Information

Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Application Note:
Flow Cytometry: 0.5-1ug/10e6 cells

Immunofluorescence: 1-2 ug/ml

Optimal dilution of the CD3 antibody for a specific application should be determined by researcher.
Background:
CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human T cell leukemia cells were used as immunogen for the CD3 antibody.
NCBI Gene ID #:
916
NCBI Official Name:
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol:
CD3E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P07766
User NOte:
Optimal dilutions for each application to be determined by the researcher