Further Information
FN1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, FINC, FN, LETS, MSF, FNZ, ED-B, GFND, GFND2
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This mAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this mAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating mAb indicating that it can be used to specifically target tumor vessels in vivo.
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
A T-lymphoma biopsy was used as the immunogen for this Fibronectin antibody.
2335
fibronectin 1
FN1
Homo sapiens
Liquid
Protein G affinity chromatography
Cancer
P02751
Optimal dilutions for each application to be determined by the researcher
