ProSci

Glial Fibrillary Acidic Protein Antibody [SPM248]

Product Code:
 
PSI-34-111
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM248
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Chicken (Gallus)
  • Human
  • Mouse
  • Porcine (pig)
  • Rabbit
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (SPM248).
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Formalin-fixed, paraffin-embedded human schwanoma stained with Glial Fibrillary Acidic Protein antibody (SPM248).

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PSI-34-111-100ug100ug£765.00
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Further Information

Additional Names:
Glial fibrillary acidic protein, GFAP, GFAP
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Western blot: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Glial Fibrillary Acidic Protein is specifically found in astroglia, making it a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Ab to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GFAP isolated from pig spinal cord was used as the immunogen for this Glial Fibrillary Acidic Protein antibody.
NCBI Gene ID #:
2670
NCBI Official Name:
glial fibrillary acidic protein
NCBI Official Symbol:
GFAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P14136
User NOte:
Optimal dilutions for each application to be determined by the researcher