ProSci

GSK-3a Antibody [SBGSK3a2] (HRP)

Product Code:
 
PSI-99-743
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[SBGSK3a2]
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Flow Cytometry
Shipping:
 
blue ice
Storage:
 
Store vial at 2-8˚C
1 / 1
GSK3 was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3?-UNLB (Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3?-HRP (Cat. No. 99-743). Proteins were visualized using chemiluminescent detection.
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GSK3 was immunoprecipitated from total cell lysates from Jurkat cells with Mouse Anti-GSK-3?-UNLB (Cat. No. 10910-01). Total cell lysates from Jurkat cells (Lane 1) and immunoprecipitate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-GSK-3?-HRP (Cat. No. 99-743). Proteins were visualized using chemiluminescent detection.

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PSI-99-743-1mL1mL£765.00
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Further Information

Additional Names:
Glycogen synthase kinase-3
Application Note:
GSK-3a Antibody [SBGSK3a2] for use in western blotting assays.
Background:
Glycogen synthase kinase-3 (GSK-3) is a protein serine kinase that phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK-3 and subsequently inhibits GSK-3 acutely leading to the stimulation of glycogen synthesis. GSK-3 signaling is performed by two isoforms, GSK-3alpha and GSK-3beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK-3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics, and cell motility.
Buffer:
Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Concentration:
Lot specific
Conjugate:
HRP
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
NCBI Gene ID #:
2931
NCBI Official Name:
Glycogen synthase kinase-3 alpha
NCBI Official Symbol:
GSK3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P49840
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
SPECIFICITY:
GSK-3alpha
Swissprot #:
P49840
User NOte:
Optimal dilutions for each application to be determined by the researcher.