ProSci

Recombinant Glypican 3 Antibody [GPC3/1534R]

Product Code:
 
PSI-34-078
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
GPC3/1534R
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody (clone GPC3/1534R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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IHC testing of FFPE human fetal liver tissue with recombinant Glypican-3 antibody (clone GPC3/1534R). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

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PSI-34-078-100ug100ug£765.00
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This product comes from: United States.
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Further Information

Additional Names:
Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5
Application Note:
Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Optimal dilution of the recombinant Glypican-3 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Glypican-3 is a proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A partial human protein was used as the immunogen for the recombinant Glypican-3 antibody.
NCBI Gene ID #:
2719
NCBI Official Name:
glypican 3
NCBI Official Symbol:
GPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P51654
User NOte:
Optimal dilutions for each application to be determined by the researcher