ProSci

GM-CSF Antibody [BVD2-21C11]

Product Code:
 
PSI-33-116
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rat
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BVD2-21C11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
 

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PSI-33-116-100ug100ug£765.00
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Further Information

Additional Names:
Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF
Application Note:
Functional Activity (Neutralization): neutralizes GM-CSF bioactivity

Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

IP: 0.5-1?g/500ug protein lysate

IHC (Frozen): 0.5-1 ug/ml for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the GM-CSF antibody to be titered up or down for optimal performance.
Background:
GM-CSF (Granulocyte/macrophage - Colony-stimulating factor) is a hematopoietic factor that is produced by activated T-cells, B-cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein was used as the immunogen for this antibody.
NCBI Gene ID #:
1437
NCBI Official Name:
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol:
CSF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology,Signal Transduction
Swissprot #:
P04141
User NOte:
Optimal dilutions for each application to be determined by the researcher