ProSci

PCNA Antibody [SPM350]

Product Code:
 
PSI-34-150
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM350
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Drosophila (fruit fly)
  • Human
  • Monkey
  • Mouse
  • Porcine (pig)
  • Rat
  • Yeast
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)
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Formalin-fixed, paraffin-embedded human tonsil stained with anti-PCNA antibody (SPM350)

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PSI-34-150-100ug100ug£765.00
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Further Information

Additional Names:
Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-PCNA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rat PCNA protein was used as the immunogen for this anti-PCNA antibody.
NCBI Gene ID #:
5111
NCBI Official Name:
proliferating cell nuclear antigen
NCBI Official Symbol:
PCNA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle
Swissprot #:
P12004
User NOte:
Optimal dilutions for each application to be determined by the researcher