Further Information
CFTR Antibody: CF, MRP7, ABC35, ABCC7, CFTR/MRP, TNR-CFTR, dJ760C5.1, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, CFTR
IF: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CFTR antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
A recombinant partial protein corresponding to amino acids 258-385 from the human protein was used as the immunogen for this recombinant CFTR antibody.
1080
cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
CFTR
homo sapiens
Liquid
Protein A affinity chromatography
Signal Transduction
P13569
Optimal dilutions for each application to be determined by the researcher