ProSci

Mitochondrial Antibody [MTL56]

Product Code:
 
PSI-33-878
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MTL56
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human pancreas stained with Mitochondrial antibody (MLT56).
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IHC testing of FFPE human pancreas stained with Mitochondrial antibody (MLT56).

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PSI-33-878-100ug100ug£765.00
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Further Information

Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

IF: 0.5-1 ug/ml

Western blot: 1-2 ug/ml

Titering of the Mitochondrial antibody may be required for optimal performance.1. FFPE staining is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
The mitochondrial marker antibody is specific for an antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and as a mitochondrial marker in subcellular fractions.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human mitochondria were used as the immunogen for this Mitochondrial antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity
User NOte:
Optimal dilutions for each application to be determined by the researcher