Connexin 32 Antibody [M12.13]

GJB1, CMTX, CX32, CMTX1

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (Frozen): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

2705

gap junction protein, beta 1, 32kDa

GJB1

Homo sapiens

Liquid

Protein G affinity chromatography

Membrane, Cancer, Signal Transduction, Neuroscience

P08034

Optimal dilutions for each application to be determined by the researcher