Further Information
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
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actin, alpha 1, skeletal muscle
ACTA1
Homo sapiens
Liquid
Protein G affinity chromatography
Cancer,Signal Transduction
P68133
Optimal dilutions for each application to be determined by the researcher