Nordic MuBio

Mouse anti FITC (Fluorescein isothiocyanate)

Product Code:
 
EXA-X1542M
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
F4/1
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Western Blot (WB)
Shipping:
 
Ship at ambient temperature freeze upon arrival
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

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CodeSizePrice
EXA-X1542M100 ug£316.00
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  • Further Information
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Further Information

Applications Description:
This antibody can be used for Western blotting (1-2 ?g/ml), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 ?g/ml) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
Background:
The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversation of fluorescein-labeled detection into immunoenzymatic assays.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Ancillary Reagents
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.
Positive Control:
Antibodies labeled with FITC
Product:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form:
FITC
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.

References

1. Balogh P; et al. 'Hapten mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody.' J. Immunol. Methods 1994, 169, 35-40.