Nordic MuBio

Mouse anti Human only p53 (a.a. 20-25)

Product Code:
 
EXA-X1495M
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BP53-12
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ship at ambient temperature freeze upon arrival
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

No additional charges, what you see is what you pay! *

CodeSizePrice
EXA-X1495M100 ug£316.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Netherlands.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
  • Further Information
  • References
  • Show All

Further Information

Applications Description:
This antibody can be used for Western blotting (0.5-1 ?g/ml), immunohistochemistry on formalin/paraffain embedded tissues (1-2 ?g/ml) and immunoprecipitation (2 ?g/mg of protein lysate). Antibody reacts with mutant as well as wild-type p53 protein. Optimal concentration should be evaluated by serial dilutions.
Background:
p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Cell Cycle
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from immunized with full length wild-type human p53 protein and mouse myeloma cells.
Positive Control:
MCF-7 cell line
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
UniProt:
P04637

References

1. Zeng, X., et al. UV but not gamma irradiation accelerates p53-induced apoptosis of teratocarcinoma cells by repressing MDM2 transcription. Cancer Res. 2000, 60, 6184-6188

2. Hussain, S.P., et al. p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology and human risk assessment. Ann. N.Y. Acad. Sci. 2000, 919, 79-85

3. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878

4. Barkte, J., et al. 'Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies.'