Nordic MuBio

Mouse anti Human Insulin

Product Code:
 
EXA-X1843M
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
E2E3
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Porcine (pig)
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ship at ambient temperature freeze upon arrival
Storage:
 
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
 

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CodeSizePrice
EXA-X1843M100 ug£316.00
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  • Further Information
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Further Information

Applications Description:
Antibody can be used for Western blotting (1-5 ?g/ml), immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-2 ?g/ml, incubate at room temp. for at least 1 hr.) and ELISA. Optimal concentration should be evaluated by serial dilutions.
Background:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes from mice immunized with full-length insulin protein and mouse myeloma cells.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
UniProt:
P01308

References

1. Roth, J., et al. 'Distribution patterns of proinsulin and insulin in human insulinomas: an immunohistochemical analysis in 76 tumors.' Virchows Arch B Cell Pathol Incl Mol Pathol 63, 51-61 (1992).

2. Nykiforuk, C.L., et al. 'Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds.' Plant Biotechnology J. 4, 77-85 (2006)

3. Lee, A.H., et al. 'XBP-1 is required for biogenesis of cellular secretory machinery of exocrine glands.' EMBO J., 24, 4368-4380 (2005)