NSJ Bioreagents

GAPDH Antibody

Product Code:
 
NSJ-RQ5051
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BG-7
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the GAPDH antibody at -20°C.
1 / 3
Western blot testing of 1) human HeLa, 2) bovine MDBK, 3) monkey COS-1 and 4) canine MDCK cell lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
2 / 3
IHC staining of FFPE human bladder cancer with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Immunofluorescent staining of human HeLa cells with GAPDH antibody (green) and DAPI nuclear stain (blue).
❮❯

Western blot testing of 1) human HeLa, 2) bovine MDBK, 3) monkey COS-1 and 4) canine MDCK cell lysate with GAPDH antibody. Predicted molecular weight ~36 kDa.
IHC staining of FFPE human bladder cancer with GAPDH antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of human HeLa cells with GAPDH antibody (green) and DAPI nuclear stain (blue).

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NSJ-RQ5051-100ul100ul£535.00
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Further Information

Application Details:
Western blot: 1:3000-1:10000,Immunohistochemistry (FFPE): 1:100-1:250,Immunofluorescence: 1:100-1:250
Application Note:
Optimal dilution of the GAPDH antibody should be determined by the researcher.
Description:
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human GAPDH was used as the immunogen for the GAPDH antibody.
Limitation:
This GAPDH antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04406