NSJ Bioreagents

HSP90 alpha/beta Antibody

Product Code:
 
NSJ-RQ5187
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
EBD-8
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the HSP90 alpha/beta antibody at -20°C.
1 / 9
IHC staining of FFPE human breast cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 9
IHC staining of FFPE human colorectal adenocarcinoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human lung cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human lymphoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE mouse brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
IHC staining of FFPE rat brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 9
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
8 / 9
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 9
Western blot testing of 1) human 293T, 2) human U-251, 3) human SiHa, 4) rat brain, 5) mouse brain and 6) mouse kidney tissue lysate with HSP90 alpha/beta antibody. Predicted molecular weight: 84-90 kDa.
❮❯

IHC staining of FFPE human breast cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with HSP90 alpha/beta antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with HSP90 alpha/beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human U-251, 3) human SiHa, 4) rat brain, 5) mouse brain and 6) mouse kidney tissue lysate with HSP90 alpha/beta antibody. Predicted molecular weight: 84-90 kDa.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ5187-100ul100ul£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the HSP90 alpha/beta antibody should be determined by the researcher.
Description:
Heat shock protein 90 (Hsp90) is one of the most common of the heat-related proteins. Hsp90 is found in bacteria and all branches of eukarya, but it is apparently absent in archaea. Whereas cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions. [Wiki]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human HSP90 alpha/beta was used as the immunogen for the HSP90 alpha/beta antibody.
Limitation:
This HSP90 alpha/beta antibody is available for research use only.
Localization:
Cell membrane, cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P07900