NSJ Bioreagents

CD45 Antibody / PTPRC

Product Code:
 
NSJ-RQ5242
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CBI-16
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the CD45 antibody at -20°C.
1 / 6
IHC staining of FFPE human spleen tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 6
Western blot testing of human Jurkat cell lysate with CD45 antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.
3 / 6
IHC staining of FFPE human placenta tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE mouse lung tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE mouse skin tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE rat stomach tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
❮❯

IHC staining of FFPE human spleen tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human Jurkat cell lysate with CD45 antibody. Expected molecular weight: 147-220 kDa depending on glycosylation level.
IHC staining of FFPE human placenta tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse lung tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse skin tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat stomach tissue with CD45 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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NSJ-RQ5242-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the CD45 antibody should be determined by the researcher.
Description:
CD45 is a protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human CD45 / PTPRC was used as the immunogen for the CD45 antibody.
Limitation:
This CD45 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08575