NSJ Bioreagents

KRT7 Antibody / Cytokeratin 7

Product Code:
 
NSJ-RQ5259
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
EBC-11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Cytokeratin 7 antibody at -20°C.
1 / 4
Western blot testing of human T-47D cell lysate with Cytokeratin 7 antibody. Predicted molecular weight ~51 kDa.
2 / 4
IHC staining of FFPE human breast carcinoma with Cytokeratin 7 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 4
Immunofluorescent staining of FFPE human HeLa cells with Cytokeratin 7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 4
Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody. Predicted molecular weight ~51 kDa.
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Western blot testing of human T-47D cell lysate with Cytokeratin 7 antibody. Predicted molecular weight ~51 kDa.
IHC staining of FFPE human breast carcinoma with Cytokeratin 7 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with Cytokeratin 7 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody. Predicted molecular weight ~51 kDa.

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NSJ-RQ5259-100ul100ul£535.00
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Further Information

Application Details:
Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence: 1:50-1:200
Application Note:
Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.
Description:
The protein encoded by the KRT7 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human Cytokeratin 7 / KRT7 was used as the immunogen for the Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P08729