Antibody Clonality:
Polyclonal
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
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![Western blot analysis of extracts of various cell lines, using SNAP25 antibody (13-354) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST. Western blot analysis of extracts of various cell lines, using SNAP25 antibody (13-354) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.](https://www.prosci-inc.com/static-images/13-354_1.jpg)
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![Immunofluorescence analysis of HeLa cells using SNAP25 antibody (13-354). Blue: DAPI for nuclear staining. Immunofluorescence analysis of HeLa cells using SNAP25 antibody (13-354). Blue: DAPI for nuclear staining.](https://www.prosci-inc.com/static-images/13-354_2.jpg)
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Further Information
SNAP25, synaptosomal-associated protein, 25 kDa, HGNC:11132, FLJ23079, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2, synaptosomal-associated protein 25
WB: 1:500 - 1:2000
IF: 1:20 - 1:100
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human SNAP25 (NP_003072.2).
6616
synaptosomal-associated protein, 25kDa
SNAP25
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Affinity purification
Cancer, Neuroscience, Signal Transduction
P60880
Optimal dilutions for each application to be determined by the researcher.