ProSci

HSP90B1 Antibody

Product Code:
 
PSI-13-356
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (13-356) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunofluorescence analysis of HeLa cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of PC-12 cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (13-356) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using HSP90B1 antibody (13-356) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-13-356-100uL100 uL£565.00
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Further Information

Additional Names:
HSP90B1, ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1).
NCBI Gene ID #:
7184
NCBI Official Name:
heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol:
HSP90B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P14625
User NOte:
Optimal dilutions for each application to be determined by the researcher.