ProSci

EIF2B5 Antibody

Product Code:
 
PSI-13-544
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using EIF2B5 antibody (13-544) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Western blot analysis of extracts of various cell lines, using EIF2B5 antibody (13-544) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

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PSI-13-544-100uL100 uL£565.00
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Further Information

Additional Names:
Translation initiation factor eIF-2B subunit epsilon, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2B5, EIF2BE
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 442-721 of human EIF2B5 (NP_003898.2).
NCBI Gene ID #:
8893
NCBI Official Name:
Translation initiation factor eIF-2B subunit epsilon
NCBI Official Symbol:
EIF2B5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 85kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q13144
User NOte:
Optimal dilutions for each application to be determined by the researcher.