ProSci

ARAP1 Antibody

Product Code:
 
PSI-13-670
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ARAP1 antibody (13-670) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of various cell lines, using ARAP1 antibody (13-670) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-13-670-100uL100 uL£565.00
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Further Information

Additional Names:
CENTD2, centaurin, delta 2, ARAP1, KIAA0782, ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1, CENTD2
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 834-1133 of human ARAP1 (NP_001128662.1).
NCBI Gene ID #:
116985
NCBI Official Name:
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
NCBI Official Symbol:
ARAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 190kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q96P48
User NOte:
Optimal dilutions for each application to be determined by the researcher.