ProSci

HADH Antibody

Product Code:
 
PSI-13-787
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using HADH antibody (13-787) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 4
Immunohistochemistry of paraffin-embedded rat spinal cord using HADH antibody (13-787) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human tonsil using HADH antibody (13-787) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody (13-787) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using HADH antibody (13-787) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat spinal cord using HADH antibody (13-787) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HADH antibody (13-787) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HADH antibody (13-787) at dilution of 1:100 (40x lens).

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PSI-13-787-100uL100 uL£565.00
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Further Information

Additional Names:
HADH, HAD, HADH1, HADHSC, HHF4, M/SCHAD, MGC8392, SCHAD, HCDH, MSCHAD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human HADH (NP_005318.3).
NCBI Gene ID #:
3033
NCBI Official Name:
hydroxyacyl-CoA dehydrogenase
NCBI Official Symbol:
HADH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q16836
User NOte:
Optimal dilutions for each application to be determined by the researcher.